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Image Search Results
Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
Article Title: Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney.
doi: 10.1093/ndt/gfp697
Figure Lengend Snippet: Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with FITC-conjugated anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
Article Snippet: Secondary goat antibodies used herein were gold-labeled anti-mouse and anti-rabbit IgG (GE Healthcare, Chalfont, St. Giles, UK), FITC-conjugated anti-rabbit IgG (pre-absorbed with rat IgG, Immuno-Biological Laboratories, Gumma, Japan),
Techniques: Labeling, Immunofluorescence, Microscopy, Incubation, Immuno-Electron Microscopy, Membrane