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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
Texas Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with <t>FITC-conjugated</t> anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.
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Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with FITC-conjugated anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney.

doi: 10.1093/ndt/gfp697

Figure Lengend Snippet: Fig. 9. SHPS-1 is predominantly localized in the podocyte of glomerulus. (A) Double-labeled immunofluorescence microscopy using anti-SHPS-1 and anti-synaptopodin antibodies. Frozen sections of normal rat kidney were fixed by PLP before incubated with the mixture of anti-SHPS-1 and anti- synaptopodin antibodies, then sequentially with FITC-conjugated anti-rabbit IgG and Texas-Red-conjugated anti-mouse IgG. Arrows indicate glomerular capillary walls. (B) Immunoelectron microscopy using anti-SHPS-1 antibody. Ultrathin sections from PLP-perfused kidney were probed with anti-SHPS-1, followed by gold-labeled anti-rabbit IgG. Symbols: P, podocyte; GBM, glomerular basement membrane; E, endothelial cell. Arrows indicate the basal membrane of foot processes, asterisks indicate slit diaphragms and arrowheads indicate the apical membranes of foot processes.

Article Snippet: Secondary goat antibodies used herein were gold-labeled anti-mouse and anti-rabbit IgG (GE Healthcare, Chalfont, St. Giles, UK), FITC-conjugated anti-rabbit IgG (pre-absorbed with rat IgG, Immuno-Biological Laboratories, Gumma, Japan), Texas-Red-conjugated anti-mouse IgG (Rockland Immunochemicals, Gilbertsville, PA, USA), HRP-conjugated anti-mouse and anti-rabbit IgG (DakoCytomation, Hamburg, Germany).

Techniques: Labeling, Immunofluorescence, Microscopy, Incubation, Immuno-Electron Microscopy, Membrane